Combined IL-2 cDNA with TNF-? cDNA genetherapy for hepatocellular carcinoma / 中国免疫学杂志

ABSTRACT

Objective:To explore antitumor effects of combined interleukin 2(IL 2)gene and tumor necrosis factor(TNF ?)gene therapy in nude mice model with hepatocellular carcinoma.Methods:Nude mice model with hepatocellualr carcinoma was established with BelH7402 cell line.Plasmid vector was used to transfect BelH7402 and nude mice model with the gene for human IL 2 or TNF ? alone or in combination by lipofectamine reagent and tumor size in nude mice model with BelH7402 carcinoma was measured before and after treatment to evaluate the response of the different treatment and control groups.Results:Tumor and BelH7402 transfected with IL 2 or TNF ? gene by liposome mediated were successful.Growth rates of BelH7402 transfected with IL 2 or TNF ? gene were not significantly changed,growth rate of tumor in nude mice model was significantly inhibited in combined IL 2 and TNF ? genetherapy group as compared with the other groups(P