The effect on sciatic nerve regeneration of non-selective antagonist of ATP receptors / 中华显微外科杂志

ABSTRACT

Objective To prove the extracellular ATP attracts the axonal regeneration after peripheral nerve injury via the ATP receptor Methods The sciatic nerve defects had been made in 10 female Sprague Danley rats and repaired by"Y" form silicon tube The single arm of the"Y" form tube were sutured with the proxma the sciatic nerve and the other two end of silicon tube sutured by paralled or cross method so as to differentiate the experimental side and control side, 1mmol/L 10 ?l ATP and 1mmol/L 10 ?m ATP+ 0 2mg/ml Suramin were injected differently into two arms of left side and 10 ?l physiological saling and 10 ?l 0 2mg/ml Suramin injected differently into two arms of right dide The silicon tube were removed after eight weeks and examine the form and number of myelinated tibers as well as the quanlity of the myelinated sheath Results In the left sides regeneration axon were tuned into the silicon tubes containing the ATP and there were not axon regeneration in the silicon tube contained the ATP and there were not axon regeneration in the silicon tube contained ATP+ Suramin In the right sides the regeneration axon were found only three tubes contained suramin and seven tubes contained physiological saline Conclusions The results demonstrated that the suramin inhibited the axon reduction of the extracellular ATP completely