Inhibition effects of epigallocatechin-3-gallste (EGCG) on cell cycle of hum an breast cancer cells MDA-MB-435 and its mechanism / 中国癌症杂志
China Oncology
;
(12)1998.
Artículo
en Chino
| WPRIM
| ID: wpr-675560
ABSTRACT
Purpose:
To study the effect of EGCG(extracts fr om green tea) on cell cycle in human breast cancer cell line and its mechanism.Methods:
Cell proliferation assay kit was used to produce the dose-response curve. Flow cytometric assay was used to evaluate the cell cycle changes on MDA-MB435 cells with and without EGCG. The expression of protein was detected by Western blot.Results:
EGCG could inhibit the proliferation of the breast canc er cells MDA-MB-435, 40 ?g/ml EGCG induced G 0 /G 1 phase arrest and the entrance to S phase. Both mRNA and protein level of p21 waf1/cip1 were up regulated in MDA-MB435 cells when treated by 40?g/ml EGCG. Conclus ionsGreen tea can inhibit the growth of human breast cancer cells, perhaps by means of p21 and therefore inhibiting the progression of the cell cycle.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Oncology
Año:
1998
Tipo del documento:
Artículo
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