Development and application of the method for the detection of human IL-6 mRNA / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Artículo
en Chino
| WPRIM
| ID: wpr-675955
ABSTRACT
Objective:
To develop and apply a method for real-time quantifying IL-6 mRNA.Methods:
By Ficoll-Hypaque density gradient centrifugation, human peripheral blood mononuclear cells(PBMC) were isolated from whole blood treated with ethylenediaminetetraacetic acid(EDTA). Total RNA extracted from the PBMC in trizol solution was reverse transcribed to cDNA, which used as templates for fluorecent real-time quantitative PCR amplification of IL-6. PCR system consists of IL-6 primer, SBY Green Ⅰ and so on.Results:
The method is wide linear range, sensitive, specific, reproducible and simple.Conclusion:
The method can accurately quantify IL-6 mRNA.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Chinese Journal of Immunology
Año:
1985
Tipo del documento:
Artículo
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