Chromosomal analysis of lung cancer by comparative genomic hybridization / 中国癌症杂志

ABSTRACT

Background and purpose:Lung cancer is thought to be caused by multiple-step carcinogenesis. Identification of the genetic alterations that occur in tumors is an important approach to understanding carcinogenesis. We identified chromosomal abnormality in lung cancer by the molecular cytogenetic techniques of comparative genomic hybridisation(CGH),the technology could help to comprehend the relationship between chromosome abnormality, different patho-types,and clinical features of lung cancer.Methods:CGH was used to detect the global genomic aberration in the fresh cancer tissue cells from 30 patients with lung cancer.Results:Chromosomal abnormality were detected in all of 30 cases with lung cancer,the altofrequent gains in 1p11-p22,5p11-p14,16p 11-P12,19q13, 19p 13,20p12,21q21 and the altofrequent losses in 5q,6p24-pter,9p31-qter,13q21-qter,14q21-qter were found in all three types of lung cancer,the marked differences of chromosomal abnormalities in three types of lung cancer were also found.Conclusions:The cytogenetic aberration exists generally in lung cancer cells,the cytogenetic aberration is the base of the initiation and progression of the lung cancer.There are some different chromosomal abnormalities between different types of lung cancer,which may serve as a marker to differential diagnosos of the three types of lung cancer.As to the progression of malignant neoplastic disease,the complexity of chromosomal abnormality is obviously elevated.Different carcinogenic agents(smoking for example)may induce different chromosomal abnormalities.