Studies on hepatic mitochondrial injury and their anti-injury capacity after superior mesenteric artery occlusion shock in the rat / 第三军医大学学报

ABSTRACT

Changes of hepatic mitochondrial respiratory function and the protective effects of several free radical scavenging enzymes or drugs on the mitochondrial functions were observed after superior mesenteric artery occlusion shock was inflicted to the rat.It was found that there was an obvious decrease of respiratory control rate (RCR) in the 1st hour after the occlusion was released and a further decrease in the 2nd hour,and P/O value decreased significantly at the same time,which indicates that mitochondrial dysfunction does occur.in the shock due to superior mesenteric artery occlusion.However,RCR ard P/O value did rot significantly decrease in the 1 st hour after occlusion releasing in the treated groups with allopurinol (ALLO),a combination of catalase (CAT) and superoxide dismutase (SOD),a combination of SOD and ALLO,and a combination of SOD,ALLO,and CAT as compared with those of the control,and were markedly higher as compared with those of the experimental group.In the 2nd hour after occlusion releasing,RCR of the ALLO treated group was significantly lower than that of the control and remained significantly higher than that of the experimental group.The average survival time of the animals was much longer in the treated groups than in the experimental group.Our findings demonstrate that free radical scavengers,SOD,CAT,and ALLO especially a combinaton of them can exert protective effects of different degrees on the mitochondrial respiratory function during a shock due to superior mesenteric artery occlusion.