Effects of total astragalosides on hepatoma cells apoptosis and wtp53 expression / 中国药理学通报

ABSTRACT

AIM\ To study whether total astragalosides(TA) induces apoptosis in human hepatoma cells and its mechanism of action. METHODS\ The human hepatoma HepG2 and Bel 7404 were cultured with TA (20,40 and 80 mg?L -1 ) for 6 days and DNA fragmentation and the expression of wtp53 protein were determined by flow cytometry. RESULTS\ HepG2 and Bel 7404 cell apoptosis induced by TA(20,40 and 80 mg?L -1 ) were found by flow cytometry. The apoptotic peak of HepG2 cells increased in dose dependent manner in the range from 4 1% to 74 5%; The apoptotic peak of Bel 7404 cells also increased from 4 5% to 50 5%. The expression of wtp53 in HepG2 cells and Bel 7404 cells treated with TA (80 mg?L -1 ) were upregulated. CONCLUSION\ TA may induce apoptosis in HepG2 cells and Bel 7404 cells, its mechanism of action might be associated with upregulating the expression of wtp53.