Computer-assisted design of TIMP-1 ribozyme / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)2000.
Artículo
en Chino
| WPRIM
| ID: wpr-677561
ABSTRACT
Objective:
To design the ribozymes to cleave human TIMP 1 mRNA, and embed them into U 6snRNA to make them stable.Methods:
Ribozymes were designed according to the “hammerhead structure” described by Symons.Computer was used to analyze the possible cleavage sites.Results:
Three ribozymes targeting the nt123, nt299 and nt353 on TIMP 1 mRNA were designed. Embedding ribozyme in U 6snRNA had little effect on its binding with the substrate.Conclusion:
Computer assisted design is indispensable in studying ribozyme. Embedding ribozymes in U 6snRNA may be a good way to solve the problems existing in ribozyme study. [
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Academic Journal of Second Military Medical University
Año:
2000
Tipo del documento:
Artículo
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