Nitric oxide preconditioning induced early protection of cardiomyocytes / 中国药理学通报

ABSTRACT

AIM To study whether nitric oxide preconditioning induce early protection of neonatal rat cardiomyocytes. METHODS Cultured neonatal rat cardiomyocytes were divided into 6 groups. ①Normal group;②NO group SNAP(500 ?mol?L -1 ) was added to cell medium 1 h before lethal hypoxia/reoxygenation (HR) injury (6 h hypoxia and 3 h reoxygenation); ③HP group Cells were cultured for 60min hypoxia followed by 30 min reoxygenation to form hypoxia preconditioning before lethal HR injury;④ L NAME+HP group Nitric oxide synthase antagonist L NAME was added during HP stage;⑤ L Arg group L arginine was added to cell medium 1h before lethal HR injury;⑥ L NAME+ L Arg group Both L NAME and L Arg were added to cell medium 1 h before lethal HR injury;⑦H/R group Cells underwent lethal HR injury without any treatment. Cardiomyocytes injury was detected by lactate dehydrogenase(LDH) activity and cell viability. RESULTS Nitric oxide preconditioning can protect cardiomyocytes by reducing LDH activity and improving cell viability ( P