Construction and identification of recombinant adenovirus shuttle plasmid pAdCMVLacZ / 第三军医大学学报
Journal of Third Military Medical University
;
(24)2003.
Artículo
en Chino
| WPRIM
| ID: wpr-678309
ABSTRACT
Objective To construct replication deficient adenovirus vector AdCMV lacZ Methods Recombinant adenovirus shuttle plasmid pAdCMVlacZ was first constructed and then lacZ cDNA cloned into Hind Ⅲ and Eco R Ⅴ of adenovirus shuttle plasmid pAdCMV by conventional methods Results The restriction enzymatic map of pAdCMVlacZ accorded with theoretic analyses and pAdCMVlacZ showed blue bacterium groups under the existence of revulsant and X gal Conclusion The adenovirus shuttle plasmid pAdCMVlacZ has been constructed successfully
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Journal of Third Military Medical University
Año:
2003
Tipo del documento:
Artículo
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