Down-regulation of the bcl-2 expression level in a human cholangiocarcinoma cell line using ribozyme / 第三军医大学学报
Journal of Third Military Medical University
; (24)2003.
Article
en Zh
| WPRIM
| ID: wpr-678322
Biblioteca responsable:
WPRO
ABSTRACT
Objective To study the role of down regulation of bcl 2 expression of human cholangiocarcinoma cells with bcl 2 mRNA cleaving ribozyme (RZ). Methods Synthetic gene of bcl 2 mRNA cleaving RZ was recombined with the expression vector of eukaryotic cells. The recombined vector was used to transfect human cholangiocarcinoma cells. The expression of bcl 2 in human cholangiocarcinoma cells was observed by immunocytochemistry and flow cytometry. Results The expression of bcl 2 in human cholangiocarcinoma cells transfected with RZ gene was lower than that of control group. Spontaneous apoptosis was observed in a few of the cells. Conclusion bcl 2 mRNA cleaving RZ can down regulate the expression of bcl 2 of cholangiocarcinoma cells notably and can also induce spontaneous apoptosis.
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WPRIM
Idioma:
Zh
Revista:
Journal of Third Military Medical University
Año:
2003
Tipo del documento:
Article