THE EFFECT OF UBIQUITIN-PROTEASOME PATHWAY ON NUCLEAR FACTOR KAPPA B ACTIVITY IN LIVER OF BURN RATS WITH SEPSIS / 解放军医学杂志

ABSTRACT

Objevtive To study the effect of ubiquitin proteasome pathway inhibition on NF ?B activity and I?B?expression as well as TNF ? secretion in the liver in burned rats with sepsis. Methods Rats were subjected to 30% full thickness scald injury, followed by intraperitoneal injection of lipopolysaccharide (LPS), was used for this experiment to mimic early sepsis after burn. Sixty Wistar rats were randomly divided into normal control group, burn sepsis group, burn sepsis with proteasome inhibitor N Acetyl leucinyl leucinyl norleucinal (ALLN) treatment group, and burn sepsis with NF ?B inhibitor Pyrrolidine Dithiocarbamate (PDTC) treatment group with 6 rats in each group. The following parameters were measured with the method indicated in parentheses NF ?B activity (electrophoretic mobility shift assay, EMSA), I?B ? expression (Western blotting), and TNF ? (Enzyme linked immunoadsordent assay, ELISA). Results These showed that NF ?B activity was markedly activated and reached its peak 1h after challenge ( P