Experimental effect of Puerarin on the formation of advanced glycation end products and expression of RAGE in the arota of diabetic rats / 中国药理学通报

ABSTRACT

AIM To investigate the effect of puerarin on the formation of advanced glycation end products (AGEs) and RAGE(receptor for advanced glycation end products)mRNA expression in the arota of diabetic rats induced by streptozotocin (STZ). METHODS Rats were injected streptozotocin into intraperitonel with a dose of 60 mg?kg -1 . Four days later, blood glucose was measured using glucose oxygen kit. The rats with over 16 7 mmol?L -1 blood glucose were considered as diabetic ones, and then divided randomly into five groups diabetic mellitus(DM), aminoguanidine(AG 0 1 g?kg -1 , ig), pue1(0 5 g?kg -1 , ig), pue2(0 25 g?kg -1 , ig) and pue3(0 125 g?kg -1 , ig). In order to set up a chronic model, we observed 12 weeks according to our past experiments. The fasting blood glucose(FBG) was and the contents of serum fructosamine (FMN) were measured using the test kit. The quantity of AGEs accumulation, the expression of RAGE in the arota was detected using fluorescence method and reverse transcription polymerase chain reaction(RT PCR). RESULTS The contents of FBG and FMN, the accumulation of AGEs in the aorta of rats treated with puerarin were lower than that of diabetic model rats( P