Study on phospholipase A_2 activation and antagonizing effect of fructose-1,6-diphosphate in endotoxin-induced acute lung injury in rabbits / 解放军医学杂志

ABSTRACT

Objective To study the changes in activity of phospholipase A_2 (PLA_2) in the course of endotoxin (ET)-induced acute lung injury (ALI) in rabbits and the antagonizing effect of fructose-1,6-diphosphate (FDP), in order to evaluate the therapeutic effect of FDP on ET-induced ALI. Methods Flap-eared white rabbits were randomly assigned to three groups control group (group A), ET challenge group (group B) and treatment group (ET challenged followed by FDP, group C). Group A animals were injected with saline (2ml/kg) as control. Group B animals were injected with ET (500?g/kg) solution followed by saline. Total amount of liquid was 2ml/kg. Group C animals were given the same amount of ET solution followed by injection of FDP (300mg/kg) solution. Total amount of liquid was also 2 ml/kg. During the experiment, respiratory rate, heart rate, blood pressure, arterial blood gases and the plasma PLA_2 activity were determined at 0h, 0.5h, 2h, 4h and 6h respectively. The rabbits were sacrificed at 6h, pulmonary PLA_2 activity was assessed, and the pathologic changes in pulmonary tissues were examined with light microscope and transmission electron microscope. Results Compared with group A, rabbits of group B manifested the typical characters of ALI after ET injection, and the PLA_2 activity in both serum and pulmonary tissue was much higher than those of group A (P