The influences of long-term cryopreservation on cell viability and lymphocyte phenotypes of peripheral blood HS/PC collections / 中国实用内科杂志
Chinese Journal of Practical Internal Medicine
;
(12)2006.
Artículo
en Chino
| WPRIM
| ID: wpr-679326
ABSTRACT
Objective To study the influences of long-term cryopreservation on nucleated cell viability,colony-form- ing units-granulocyte-macrophage(CFU-GM)-forming capability and Lymphocyte immune-phenotypes of pe- ripheral blood hematopoietic stem/progenitor cell(HS/PC)collections.Methods Fresh samples(n=12)were as control,frozen samples were divided into two groupsgroup 1(cryopreserved for 3-5 years,n=26)and group 2 (cryopreserved for 5-7 years,n=9).Flow cytometry(FCM)method based on 7 AAD or caspase-3staining was ap- plied to assess the degree of cell necrosis or apoptosis in cryopreserved and red-cell lytic collection samples.Lympho- cyte immune-phenotypes(CD3/CD4/CD8/CD19/CD16+56,CD4/CIM5RA/CIMSRO,CD8/CD28)were also de- tected by FCM.CFU-GM culture was done to study the hematopoietic activity.Results Percentages of necrosis and apoptosis cells of the control were both less than the groupl and group2(P
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Practical Internal Medicine
Año:
2006
Tipo del documento:
Artículo
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