Expression changes of protein tyrosine phosphatase 1B during differentiation of 3T3-L1 preadipocytes / 第二军医大学学报

ABSTRACT

Objective:To observe the expression changes of protein tyrosine phosphatase 1B(PTP1B)mRNA and protein during the differentiation of 3T3-L1 preadipocytes,so as to explore the relationship between PTP1B and adipocytes differentiation.Methods:3T3-L1 preadipocytes were cultured in vitro and were induced to differentiate into mature adipocytes; the differentiation of adipocytes was assessed through detecting expression of peroxisome proliferator-activated receptor-gamma 2(PPAR?2)mRNA by RT-PCR and oil red O staining.Expression of PTP1B in adipocytes was detected by RT-PCR and Western blot during differentiation.Results:With the progression of 3T3-L1 cell differentiation,oil red O staining showed that the lipid droplets increased gradually to 90% of the vision field;meanwhile,the expression of PPAR?2 also increased gradually, suggesting the proliferation and maturation of the preadipocytes.The expression of PTP1B mRNA and protein decreased in 3T3-L1 preadipocytes with their differentiation and maturation;the expression reached the lowest level in mature adipocytes. Conclusion:Expression of PTP1B mRNA and protein decreases during the differentiation of preadipocytes,indicating a role for PTP1B in the maturation of adipocytes.