The Effect of High Glucose on Renin-Angiotensin System (RAS) in Podocytes / 대한신장학회잡지
Korean Journal of Nephrology
;
: 903-912, 2006.
Artículo
en Coreano
| WPRIM
| ID: wpr-68013
ABSTRACT
The renin-angiotensin system (RAS) plays an important role in the pathogenesis of diabetic nephropathy. Recently, the activation of local RAS in mesangial cells by high glucose has been reported. However, little is known about the changes of RAS in podocytes under diabetic conditions. In this study, we examined whether RAS activation was induced in high glucose- stimulated podocytes. Immortalized mouse podocytes were exposed to medium containing 5.6 mM glucose (NG), NG+24.4 mM mannitol, or 30 mM glucose(HG). mRNA and protein expression of RAS components were determined by real time-PCR and Western blot, respectively. Angiotensin I (AI) and angiotensin II (AII) concentrations, angiotensin-converting enzyme (ACE) levels, and renin activity were also determined. Angiotensinogen (AGT) mRNA expression was significantly increased in HG-stimulated podocytes. In addition, AI and AII concentrations were significantly higher in HG-treated cell lysates and in their conditioned media. However, there were no differences in renin activity and ACE levels among the groups. AII type 1 receptor (AT1R) mRNA and protein expression were also increased by 288% (p<0.01) and 170% (p< 0.05) in HG-stimulated podocytes compared to NG- treated cells. In conclusion, HG induced AGT mRNA expression, resulting in increases in AI and AII levels. These findings suggest that increased AII production along with increased AT1R expression in podocytes under diabetic conditions may well be considered as factors actively involved in the pathogenesis of diabetic nephropathy.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sistema Renina-Angiotensina
/
ARN Mensajero
/
Angiotensina I
/
Angiotensina II
/
Angiotensinógeno
/
Western Blotting
/
Renina
/
Medios de Cultivo Condicionados
/
Nefropatías Diabéticas
/
Células Mesangiales
Límite:
Animales
Idioma:
Coreano
Revista:
Korean Journal of Nephrology
Año:
2006
Tipo del documento:
Artículo
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