SENSETIVITY AND SPECIFICITY OF HOMEMADE ANTIBODY AGAINST DOPAMINE / 解剖学报

ABSTRACT

Dopamine is one of the bioactive amines which regulate many physiological functions. In order to study its localization and quantification in tissues with immunohistochemistry and radioimmunoassay, authors immunised New Zealand rabbits with dopamine-BSA (bovine serum albumin) complex and succeeded in the production of the specific antiserum. The antiserum was tested on routine paraffin sections of Bouin's fixed guinea-pig digestive tract with the ABC technique. The titre of the antiserum was 11000-12000. Results of substitution, absorption and cross-absorption tests proved that the positive staining obtained with the antiserum demonstrated the specific immunoreaction between the antibody to dopamine and the antigen in the tissues.