Determination of notoginsenoside R_1 and ginsenoside Rg_1, Rb_1 in Xuesaitong Dripping Pills by RP-HPLC gradient elution method / 中成药

ABSTRACT

Objective: A new HPLC gradient elution was studied for the determination of notoginsenoside R 1, ginsenoside Rg 1 and Rb 1 in Xuesaitong Dripping Pills. Methods:C 18 column was used with a mobile phase of acetonitrile water 0～15 min. of (20∶80～40∶60) gradient elution, post time was 5 min, the wavelength of detecter was set at 203nm. Results: The linear range of R 1, Rg 1 and Rb 1 was 1.02～9.18?g,4.8～ 43.4?g and 4.6～41.6?g, respectively, the average recovery rate was 101.0% ( RSD =3.18%), 99.5%( RSD =3.02%) and 100.0%( RSD =1.19), respectively. Conclusion: The method is rapid, accurate, sensitive and relieble. The results show that the method can be used to control quality of products.