Influence of Astragalus Injection on expression of myocardial perforin with murine actute myocarditis caused by Coxsackievirus B3 / 中成药

ABSTRACT

Objective: To investigate the effect and mechanism of Astragalus Injection (Radix Astragali) on acute murine myocarditis caused by Coxsackievirus B 3. Methods: Acute viral myocarditis was induced in 24 Balb/c mice by injection of Coxsackievirus B 3m (CVB 3m ) intraperitoneally. Half of the mice were administered Astragalus Injection(10g/kg?d). Heart and blood samples were obtained from all mice on the 8th day after CVB 3m infection for histopathological study, detection of myocardial perforin expression by reverse transcription polymerase chain reacton(RT PCR) and measurement of serum CK MB level, respectively. Results: (1) Astragalus treated mice showed a significant reduction in myocardial lesion compared with that in untreated mice. (2) Myocardial perforin expression by semi quantitative RT PCR in Astragalus treated mice was much lower than that in controls(1.10?0.07 vs 1.31?0.12, P