Detection of Virulent Gene Distribution of Diarrheagenic Escherichia Coli (DEC)

ABSTRACT

Introduction: There are 5 identified DEC including EAEC, EHEC, EIEC, EPEC and ETEe. Virulent genes (for adherrnee, toxin, antibiotic resistance ...) play important roles in pathogenesis of DEe. Detection of DEC is very important in diagnosis, epidemiology survey and vaccine development. \r\n', u'Objectives: Detection of virulent gene distribution of DEC and non - DEe.\r\n', u'Object and methods: 161 strains of DEC (EAEC, EIEC, EPEC, TEC) and 100 strains of non - DEC were subjected to this study. PCR with specific primers were used to test these genes. \r\n', u'Results: EAEC that accounted for 50% of DEC, was identified and isolated. Aap gene was the highest prevalence in EAEC (96.5%), followed by aggR (79.1 %) and astA (60.5%). 37.2% of the strains harbor all three genes. None of strains had PCR results negative for these 3 genes. ETEC, EPEC and EIEC had aap, and astA gene at the prevalence from 7% to 72.7%. The highest prevalence of aap was seen in EIEC 72.7%), aggR in EIEC (45.5%), and astA in ETEC (50%). 14% of non - DEC had aggR and more than 30% of E. coli had aap and astA gene. \r\n', u'Conclusion: EAEC is prevalent at 50% among Diarreagenic E. coli. Aap is the most prevalent and the most commonly seen among EAEC isolates. The other three genes are at different prevalence. The findings contribute towards the vaccine development against diarrhea caused by E. coli. \r\n', u'