ISOLATION AND CULTURE OF NEURAL STEM CELLS FROM BRAIN OF RAT / 解剖学报
Acta Anatomica Sinica
;
(6)1955.
Artículo
en Chino
| WPRIM
| ID: wpr-682075
ABSTRACT
Objective To investigate the effect of optimized conditions on proliferation and differentiation of the neural stem cells(NSCs) from different age brain of rat in vitro. Methods The brain tissues from embryonic(E12~E15d),newborn(1~2?d) hippocampus and olfactory bulb from adult of rat were isolated.After proliferation in conditional culture medium including growth factor and induced differentiation,the cell type specific antibodies were used for the immune fluorescence staining to identify the cells. Results A lager scale NSCs were obtained from brain tissue and could proliferate in conditional culture in vitro. They could self renew and generate both nerve and glia.Conclusion\ The NSCs got from rat brain can be used for basic and clinical research.\;[
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
Acta Anatomica Sinica
Año:
1955
Tipo del documento:
Artículo
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