Determination of chlorogenic acid in different processed crude drugs of Lonicera fulvotomentosa by HPLC / 中草药

ABSTRACT

Object To compare the contents of chlorogenic acid in the different processed crude drugs of Lonicera fulvotomentosa Hsu et S.C. Cheng. Methods The contents of chlorogenic acid were deter mined by HPLC. Chromatographic conditions included Hypersil C 18 column and the mobile phase consisting of a mixture of acetonitrile 0 4% phosphoric acid (13∶87). Chlorogenic acid was detected at 327 nm wavelength. Results The contents of chlorogenic acid were 0 54%, 0 81%, 0 57% in the sun cured flower bud, white bloom, gold flower in L. fulvotomentosa and 2 14%, 1 65%, 2 04% in the steaming sun cured crude drugs, respectively. Conclusion The contents of chlorogenic acid in the steaming sun cured crude drugs are higher than those in the sun cured crude drugs.