Determination of volatile components in Liangmian Honghua Ointment / 中成药

ABSTRACT

AIM: To establish a method for determination of volatile components(? pinene、? pinene、camphor、menthol、isoborneol、borneol、cinnamaldehyde、paeonol and laurocapram)in Liangmiao Honghua Ointment (Radix Zanthoxyli, Flos Carthami, Radix Notoginseng, paeonol, etc.). METHODS: Nine volatile components were isolated and determined on ZB WAX capillary column(30 m?0.25 mm?0.25 ?m) by GC. The column temperature was raised by program, 50 ℃(3 min)→30 ℃?min -1 →120 ℃(5 min)→10 ℃?min -1 →150 ℃ (1 min)→60 ℃?min -1 →230 ℃(12 min). Split stream sampling was applied,split ratio was 20∶1; injection port temperature was 210 ℃; detector FID, temperature at 250 ℃. RESULTS: The nine volatile components were isolated well. The linearities were fine with the recoveries of 97%-100%, respectively. CONCLUSION: The method is convenient,rapid and accurate.It can be used to control the quality of Liangmian Honghua Ointment.