SCREENING OF CURVULARIA LNATA WITH THE ABILITY OF BIOCONVERTING RSA TO HYDROCORTISONE / 微生物学通报

ABSTRACT

A strain C4 with ability of bioconversion from steroid RSA to hydrocortisone was selected fron the soil sample. It was identifide as Cuvularia Iunata according its morphological characters of clump and individuals.The optimum culture medium was established by orthogonal experimentsglucose 10g,soybean powder 10g,water 1000mL, pH6.5.Determined by silica gel chromatography and high performance liquid chromatography, the bioconversion ratio of hydrocortisone was 34%.At the same tine, the percentage change of RSA,hydrocortisone and other byproducts were measured during the course of bioconversion.