STUDIES ON LIQUID CULTURE CONDITIONS OF SE-ACCUMULATING AGARICUS BLAZEI / 微生物学通报

ABSTRACT

The conditions of Se accumulating Agaricus blazei in submerged culture with shake flask were studied in this paper, experimental results showed that the composition of the cultural medium was as follow corn starch 30g/L,glucose 20g/L,(NH 4) 2SO 4 2g/L, yeast extract 3g/L, KH 2PO 4 2g/L, MgSO 4?7H 2O 1g/L and VB 1 100mg/L,that strains domesticated in slant with 1mg/L Se, 250mL conical bottle contained with 80mL liquid cultural medium, pH 7 , 80r/min and 12 days of cultural period were the optimum cultural conditions,and that on such conditions , dry weight of the mycelium reached 18 6g/L,Se accumulated in the mycelium reached 55 32?g/g and Se accumulation rate reached 5 14%