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The roles of NF-?B in corticosterone-injured hippocampal neurons / 医学研究生学报
Journal of Medical Postgraduates ; (12)2003.
Artículo en Chino | WPRIM | ID: wpr-684184
ABSTRACT

Objectives:

To observe the roles of nuclear factor ?B(NF ?B) in corticosterone injured hippocampal neurons.

Methods:

Two groups of primary hippocampal neurons were cultured for 8~10 days. One group was treated with 1?10 -5 mol/L corticosterone. The total cellular nuclear protein levels were extracted at 0.5,1,2 and 4 hours, respectively. Electrophoresis mobility shift assay (EMSA) was used to detect the expression of NF ?B activation. Another group was treated with ?B decoy DNA(25 ?mol/L) to inhibit the activity of NF ?B for 2 hours before 1?10 -5 mol/L corticosterone was intervened. MTT was used to observe the effects of corticosterone induced neurons injury after 6,12,24 and 48 hours.

Results:

The activity of NF ?B became significantly lower than that in the control group after corticosterone acted on neurons for 1 hour. As time went on, its effects became more conspicuously. The injury of hippocampal neurons aggravated when the activity of NF ?B was inhibited by ?B decoy DNA.

Conclusions:

NF ?B plays a protective role in corticosterone induced hippocampal neuron injury.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Journal of Medical Postgraduates Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Journal of Medical Postgraduates Año: 2003 Tipo del documento: Artículo