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Application of two bacteria colony-PCR methods in the screening of phage antibody library / 医学研究生学报
Journal of Medical Postgraduates ; (12)2003.
Artículo en Chino | WPRIM | ID: wpr-684635
ABSTRACT

Objective:

To value the application of two bacteria colony-PCR methods in the screening of phage antibody library.

Methods:

Five positive monoclonal bacterium were respectively suspended in either deionized water or 0.1% Triton X-100, and then boiled to be used as template in PCR. . The DNAs products of PCR were extracted and digested by two enzymes, and then determined by electrophoresis.

Results:

The inserted genes were detected in all the 5 clones after PCR and enzyme digestion .

Conclusion:

Bacteria colony-PCR can be used in screening positive recombinant colonies. The bacteria colony-PCR method with bacteria colonies suspended in deionized water is valuable in large scale positive recombinant bacterium screening.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio diagnóstico / Estudio de tamizaje Idioma: Chino Revista: Journal of Medical Postgraduates Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio diagnóstico / Estudio de tamizaje Idioma: Chino Revista: Journal of Medical Postgraduates Año: 2003 Tipo del documento: Artículo