Study on the Bombardment Factors for Kentucky Bluegrasss (Poa pratensis L.) / 中国生物工程杂志
China Biotechnology
;
(12)2006.
Artículo
en Chino
| WPRIM
| ID: wpr-685122
ABSTRACT
Embryonic callus of Kentucky bluegrass were used as material for genetic transformation with the DREB1A gene by particle bombardment. Parameters of biolistic bombardment were studied. The optimal methods showed as follows plasmid DNA are coated by Ca(NO_3)_2 and PEG4000, 1?m golds are the vectors of plasmid DNA, 6 cm bombardment height distance, 1 time and without osmotic treatment are favorable to transgenic efficiency. The concentration hygromycin (Hy) , which is the selection mark for cultivar‘Baron’ is 100mg/L.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Biotechnology
Año:
2006
Tipo del documento:
Artículo
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