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Analysis of Cis-acting Elements on Respiratory Mucin MUC5AC Expression and Regulation in Pulmonary A549 Cell Line / 中国生物工程杂志
China Biotechnology ; (12)2006.
Artículo en Chino | WPRIM | ID: wpr-685321
ABSTRACT

Objective:

To study the mechanisms of neutrophil elastase (NE) induced expression of respiratory mucin MUC5AC.

Methods:

Using gene recombination techniques,four luciferase reporter gene plasmids containing different length of human MUC5AC gene promoter were constructed.Site-directed mutagenesis technique was used to establish mutants of Sp-l and NF-?B site in MUC5AC gene promoter; the relative luciferase activities were detected in the transfected human pulmonary A549 cells.

Results:

Series of luciferase reporter gene containing different sequences of human MUC5AC promotor were constructed successfully.NE could increase the expression of luciferase reporter gene plasmid containing -1300bp,-689bp and-324bp version of MUC5AC promoter in the transfected A549 cells (P

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: China Biotechnology Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: China Biotechnology Año: 2006 Tipo del documento: Artículo