Study of Expression of Pneumococcal Surface Protein and Immunogenicity / 中国生物工程杂志
China Biotechnology
; (12)2006.
Article
en Zh
| WPRIM
| ID: wpr-685823
Biblioteca responsable:
WPRO
ABSTRACT
The specific fragment of Pneumococcal surface protein A(PspA)and Pneumococcal Surface Adhesin A(PsaA)gene was amplified by PCR from Streptococcus pneumonia 5 and Streptococcus pneumonia 19.The amplified fragnent of PspA and PsaA gene was ligated into pET-27b(+)vector and transformed into BL 21 E.coli for expression and obtain the expressive production of PspA and PsaA.Induced by IPTG,the expression level was as high as 75 % of the total disolube protein.The result showed that the recombinant plasmid could express a specific 75 kDa and 37 kDa fusion protein in E.coli BL 21,which showed the good immunogenicity and a broadly cross reactivity with the other serotypes.
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WPRIM
Idioma:
Zh
Revista:
China Biotechnology
Año:
2006
Tipo del documento:
Article