Application of Thermal Shift Assays in Purification of Recombinant TBEV Serine Protease / 中国生物工程杂志
China Biotechnology
;
(12)2006.
Artículo
en Chino
| WPRIM
| ID: wpr-686402
ABSTRACT
Thermal Shift Assays was applied for high-throughput screening optimized buffer system used in the gel filtration chromatography for purification of recombinant TBEV serine protease. The optimization included kinds of salt and pH. It was optimized new buffer remarkably improved the quality of the purified TBEV serine protease by increasing its monomer from 75% to 99%. This result may improve future crystal studies of this protein.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Biotechnology
Año:
2006
Tipo del documento:
Artículo
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