Vancomycin-based fluorescent enzyme-linked immunoabsorbent assay for detection of Staphylococcus aureus / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1510-1517, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-687668
ABSTRACT
In the study, fluorescent enzyme-linked immnoabsorbent assay for detection of Staphylococcus aureus was established with IgG from pig as capture antibody and quantum dot nanobeads (QBs) labeled vancomycin (QB-Vans) as testing antibody. Quantum dot of about 100 nm partical size nanobeads were prepared and linked with vancomycin. The optimum concentrations of salt ions were 0.01 mol/L, and the optimum pH was 6.0. Under the optimum conditions, the detection sensitivity for S. aureus was 10⁴ CFU/mL, and there was no cross-reaction with other pathogenic bacteria. Thus, the method could be used for rapid screening of S. aureus, for the clinical monitoring and foodborne pathogens detection.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2018
Tipo del documento:
Artículo
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