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Molecular identification of cucumber mosaic virus in woad (Isatis tinctoria) with mosaic disease in Beijing / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2242-2245, 2018.
Artículo en Chino | WPRIM | ID: wpr-690504
ABSTRACT
To detect possible pathogenic virus(es) in woad (Isatis tinctoria) cultivated at Institute of Medicinal Plant Development in Beijing, reverse transcription(RT)-PCR was performed using total RNA of symptomatic woad leaves with primers for poty-, polero-, tobamovirus, broad bean wilt virus 2(BBWV2) and cucumber mosaic virus (CMV). A 657 bp fragment was amplified from symptomatic woad using CMV primers. Sequencing and BLAST analysis indicated that this fragment shared 99% nucleotide identity and 100% amino acid identity with CMV-Vi isolate. The isolate was named CMV-Isatis tinctorial (CMV-It). Phylogenetic analysis based on nucleotide sequences of CP genes showed that CMV-It clustered with CMV-K and belonged to subgroup I. To our knowledge, this is first identification of CMV in woad by RT-PCR and the CP gene was analyzed. This work provided data for research and control of woad mosaic disease.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio diagnóstico Idioma: Chino Revista: China Journal of Chinese Materia Medica Año: 2018 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Tipo de estudio: Estudio diagnóstico Idioma: Chino Revista: China Journal of Chinese Materia Medica Año: 2018 Tipo del documento: Artículo