Inhibitory Effect of miR-125b Down-Regulation on Proliferation of Leukemia Cell K562 / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore inhibitory effect of miR-125b down-regulation on proliferation of leukemia cell K562.</p><p><b>METHODS</b>miR-125b inhibitor and miR-125b NC were transfected into K562 cells by liposome Lipofectamine2000, the cell viability was measured by MTT assay, cell cloning ability was detected by agarose cloning assay, cell cycle was measured by flow cytometry. The expression of BCL-2, BCL-2 homology antagonist/liller 1(BAK1), p53 and p53 up-regulated modulator of apoptosis (Puma) was measured by Western blot.</p><p><b>RESULTS</b>Compared with miR-125b NC, the expression of miR-125b was down-regulated (P<0.01), the cell viability and cell cloning ability were reduced (P<0.01), the cell cycle was arrested in the G phase (P<0.01), the expression of BCL-2 were down-regulated (P<0.01), the expression of BAK1, p53 and Puma were up-regulated in miR-125b inhibitor group (P<0.01).</p><p><b>CONCLUSION</b>Down-regulation of miR-125b can inhibit K562 cell proliferation via down-regulating the expressions of BCL-2 and up-regulating the expression of BAK1, p53 and Puma.</p>