Influence of apatinib combined radiotherapy on HeLa cell cycle and apoptosis in vitro / 安徽医科大学学报

ABSTRACT

Objective To observe the effect of apatinib (APA) combined with radiotherapy on cell cycle and apoptosis of cervical cancer HeLa cells in vitro. Methods HeLa cells in logarithmic growth phase were divided into control group, drug group, radiotherapy group and joint group. Cell cycle and apoptosis were detected by flow cytometry, and the changes of HeLa cell cycle and apoptosis after radiotherapy were analyzed. Results Retardant of G0/G1 phase for joint group was obviously higher than control and radiotherapy group (P ＜ 0. 05). S phase percentage of joint group was minimum when compared with control group (P ＜0. 05), while there was no significant difference when compared with radiotherapy group. Apoptosis rate of joint group was higher than control group and drug group (P ＜ 0. 05 ). Conclusion APA combined with radiotherapy shows significant G0/G1 phase cell cycle arrest, but no significant induction of apoptosis.