Peripheral blood expression levels of cytokines in newly diagnosed patients with diffuse large B-cell lymphoma and their clinical significances / 白血病·淋巴瘤

ABSTRACT

Objective To detect the expression levels of cytokines in peripheral blood of patients with diffuse large B-cell lymphoma (DLBCL), and to explore their clinical significances. Methods The levels of interleukin-1β (IL-1β), IL-2R, IL-6, IL-8, tumor necrosis factor (TNF) and IL-10 in peripheral blood of 38 newly diagnosed patients with DLBCL and 20 healthy adults in Anqing Municipal Hospital from April 2013 to January 2017 were detected and analyzed by immunofluorescence method. Results The levels of IL-2R, IL-6, IL-8, TNF and IL-10 in DLBCL group were significantly higher than those in healthy control group (all P< 0.05). The levels of IL-2R and TNF in stage Ⅲ-Ⅳ group [(5985±26) U/ml and (42.2±5.8) pg/ml)] were higher than those in stageⅠ-Ⅱ group [(3672 ±28) U/ml and (30.4 ±2.6) pg/ml] (t values were 34.861 and 28.451; P values were 0.023 and 0.038 respectively). The levels of IL-2R and IL-10 in the high-risk group (IPI 4-5) [(6322±36) U/ml and (77±7) pg/ml] were higher than those in the low-risk group (IPI 0-1) [(2567± 32) U/ml and (50±5) pg/ml)] (t values were 58.327 and 24.264; P values were 0.001 and 0.041 respectively). IL-2R, IL-6 and IL-10 levels in patients with B symptom group [(6234±26) U/ml, (38.1±2.3) pg/ml, (90± 10) pg/ml] were higher than those in patients without B symptom group [(6234 ±26) U/ml, (38.1 ±2.3) pg/ml, (90±10) pg/ml) (t values were 32.263,24.321, 36.529; P values were 0.027, 0.043, 0.020). Conclusion The levels of IL-2R, IL-6, IL-8, TNF and IL-10 in DLBCL patients are higher than those in healthy controls, and the levels of cytokines are correlated with the clinical characteristics of DLBCL patients.