Effect of sub-minimal inhibitory concentration piperacillin/tazobactam on biofilm formation of Escherichia coli isolates / 重庆医学

ABSTRACT

Objective To study the effect of sub-minimal inhibitory concentration(sub-MIC) piperacillin/tazobactam on the biofilm formation of Escherichia coli to provide the theoretical basis for clinical anti-infection treatment.Methods The minimal inhibitory concentration of E.coli was detected by broth microdilution method.The biofilm formation ability was analyzed by using the 96-well crystal violet staining method.The bacterial adhesion ability was determined by adopting the flat colony counting method.The effect of sub-MIC piperacillin/tazobactam on the motility of E.coli was determined by adopting the swimming movement.Results Sub-MIC piperacillin/tazobactam could significantly inhibit the biofilm formation of E.coli(P＜ 0.05).In addition,subMIC piperacillin/tazobactam not only significantly reduced bacterial adhesion,but also decreased the bacterial motility.Conclusion Sub-MIC piperacillin/tazobactam might inhibit the biofilm formation of E.coli by inhibiting its adhesion and motility.