GC/MS based metabonomie study of neonatal hepatitis syndrome / 国际儿科学杂志

ABSTRACT

Objective Early detection is the most effective way to improve the clinical outcome of biliary atresia(BA).Emerging metabolomics provides a powerful platform for discovering novel biomarkers and biochemical pathways to improve early diagnosis.The aim of this study is to find the potential biomarkers to distinguish BA from neonatal hepatitis syndrome(NHS) by using a metabolomics method.Methods We comprehensively analyzed the serum metabolites in a total of 26 blood samples from patients with BA or neonatal hepatitis syndrome(NHS) and from normal individuals using advanced metabolomic approaches.Results The levels of propanoic acid,hexadecanoic acid,eicosanoic acid,octadecenoic acid and cholesterol significantly increased in the BA group.Conclusion The levels of L-Tyrosine(Tyr)were reduced in the BA group compared to those in the NHS group,but still higher than the normal controls.The levels of L-Proline(Pro) in the NHS group were significantly elevated compared to those in the BA group.And at the same time,we find 5 patients with cirin deficiency.This study demonstrates the possibility of metabolomics as non-invasive biomarkers for the early detection of BA and also provides new insight into pathophysiologic mechanisms for BA.