Evaluation of monocytes and dendritic cells changes in peripheral blood of diffuse large B-cell lymphoma patients before and after GM-CSF treatment / 国际检验医学杂志

ABSTRACT

Objective To evaluate the changes of monocytes,dendritic cells(DC),myeloid dendritic cells (mDC)and plasma dendritic cells(pDC)of peripheral blood in diffuse large B-cell lymphoma(DLBCL)pa-tients after 3 courses of GM-CSF treatment.Methods 33 patients diagnosed with DLBCL in the hospital from October 2015 to February 2016 were enrolled in the study.All the patients were treated with GM-CSF for 3 courses.Before each course,3 mL venous blood samples were extracted and the monocytes,dendritic cells,my-eloid dendritic cells and plasma dendritic cells were analyzed by using flow cytometer within 24 h.All the data was analyzed with SPSS17.0 software.Results After 3 courses′ treatment,the monocytes decreased signifi-cantly,from(9.85 ± 2.31)% to(3.67 ± 0.78)%.Dendritic cells increased significantly,from(0.24 ± 0.04)% to(0.58 ± 0.13)%.mDCs increased significantly from(0.11 ± 0.02)% to(0.36 ± 0.08)%,however, pDCs only increased from(0.13 ± 0.03)% to(0.21 ± 0.04)% with no statistically significance.Conclusion After 3 treatment courses with GM-CSF,the monocytes decreased obviously while DCs especially mDCs in-creased obviously in DLBCL patients.GM-CSF might activate the anti-tumor immunity of DLBCL patients.