Effects of knockdown of Stat5 on invasion and epithelial mesenchymal transition of thyroid carcinoma TT cells / 国际肿瘤学杂志

ABSTRACT

Objective To investigate the effects of knockdown of signal transducer and activator of transcription 5 (Stat5) on invasion and epithelial mesenchymal transition (EMT) of thyroid carcinoma TT cells.Methods Thyroid carcinoma TT cells were cultured and divided into Stat5-small interfering RNA (siRNA) group and NC-siRNA group,transfected with Stat5 siRNA and negative control NC-siRNA respectively.After transfection,the numbers of invasive cells were determined by Transwell model and the expressions of matrix metalloproteinase (MMP) family genes,EMT marker genes,angiogenesis genes in the cells were determined by fluorescent quantitative PCR.Results The numbers of invasive cells in Stat5-siRNA group were significantly lower than those in NC-siRNA group (42.39 ± 5.82 vs.64.41 ± 8.49,t =-4.784,P =0.001;51.23 ± 6.38 vs.78.54 ± 9.52,t =-5.329,P ＜ 0.001;60.35 ± 8.35 vs.98.42 ± 11.25,t =-6.076,P ＜0.001) after the transfection of siRNA for 12 hours,18 hours and 24 hours.Twenty-four hours after transfection of siRNA,the mRNA expressions of MMP1,MMP2,MMP9,MMP13,N-cadherin,Vimentin,vascular endothelial growth factor (VEGF),basic fibroblast growth factor (bFGF),angiogenin (Ang)-1 and Ang-2 in Stat5-siRNA group were significantly lower than those in NC-siRNA group (0.42 ± 0.07 vs.1.03 ± 0.15,t =-8.240,P＜0.001;0.35 ±0.06 vs.1.01 ±0.13,t=-10.307,P＜0.001;0.29±0.05 vs.1.05± 0.18,t=-9.097,P＜0.001;0.54±0.08 vs.0.98±0.12,t=-6.822,P＜0.001;0.38 ±0.06 vs.1.04±0.18,t=-7.778,P＜0.001;0.29±0.04 vs.1.06±0.12,t=-12.612,P＜0.001;0.36±0.07 vs.1.06 ±0.14,t=-10.000,P＜0.001;0.43 ±0.08 vs.0.97 ±0.12,t=-8.372,P＜0.001;0.25 ± 0.03 vs.1.03±0.12,t=-14.100,P＜0.001;0.19±0.03vs.0.99±0.13,t=-13.408,P＜0.001).The mRNA expression of E-cadherin was significantly higher than that in NC-siRNA group (2.88 ± 0.42 vs.0.98 ± 0.12,t =9.726,P ＜ 0.001).Conclusion Knockdown of Stat5 inhibits the invasion and EMT of thyroid carcinoma TT cells.