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Characterization of Echinostoma cinetorchis endoribonuclease, RNase H
Article en En | WPRIM | ID: wpr-69358
Biblioteca responsable: WPRO
ABSTRACT
Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3′-O-P bond of RNA in a DNA-RNA duplex, producing 3′-hydroxyl and 5′-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.
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Texto completo: 1 Índice: WPRIM Asunto principal: Parásitos / Ribonucleasas / Trematodos / ARN / Oligonucleótidos Antisentido / ADN Complementario / Ribonucleasa H / Dominio Catalítico / Echinostoma / Endorribonucleasas Idioma: En Revista: The Korean Journal of Parasitology Año: 2017 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Parásitos / Ribonucleasas / Trematodos / ARN / Oligonucleótidos Antisentido / ADN Complementario / Ribonucleasa H / Dominio Catalítico / Echinostoma / Endorribonucleasas Idioma: En Revista: The Korean Journal of Parasitology Año: 2017 Tipo del documento: Article