Differences of cellular apoptosis in lungs between C57BL/6J and C3H/HeN mice in the course of radiation induced pulmonary injury / 军事医学

ABSTRACT

Objective To explore the difference of cellular apoptosis levels in lungs between C 57BL/6J and C3H/HeN mice in the course of radiation induced pulmonary injury (RPI).Methods Sixty C57BL/6J mice and another sixty C3H/HeN mice were randomly divided into control group and irradiation group respectively .The 60 Co γray irradiation method was used to establish an RPI model with 20 Gy per irradiation.Lungs were obtained on 1 d, 3 d, 7 d, 1 month(m) and 3 m after irradiation, and then the apoptosis and expressions of γH2AX and AIF were detected by in situ terminal labeling and immunoblotting .Results 1 and 3 d after irradiation , many apoptotic cells , mainly apoptotic epithelial cells , could be seen in lungs and apoptotic cells in the lungs of C 57BL/6J mice outnumbered those of C3H/HeN mice.At one month after irradiation, there was no significant difference of cell apoptosis between these two groups of mice .Three months after irradiation, there were fewer apoptotic cells in lungs of C 57BL/6J mice than in C3H/HeN mice.C57BL/6J mice had a higher γH2AX expression in lungs than C3H/HeN mice did at 1 -7 days after irradiation.However, the expression ofγH2AX in lungs of C3H/HeN mice was higher than that of C57BL/6J mice at 3 m after irradiation.The expression of AIF in lungs of C3H/HeN mice was lower than that of C57BL/6J mice at 1 d, 3 d and 1 m,while the expression of AIF in lungs of C3H/HeN mice was higher than that of C57BL/6J mice at 3m after irradiation.Conclusion In the course of RPI, the occurence of DNA damage and cell apoptosis is different in lungs of C 57BL/6J mice and C3H/HeN mice.