Antibody Response of Mouse Splenocytes Using Mixture of Supernatants of Thymocytes, Adherent and Non-adherent Splenocytes: In-vitro Immunization-II
Journal of Korean Medical Science
;
: 25-31, 1990.
Artículo
en Inglés
| WPRIM
| ID: wpr-69573
ABSTRACT
We adapted one of the in-vitro immunization methods to induce antibody responses and confirmed the success of the immunization by enzyme-linked immunosorbent assay (ELISA) without hybridization. We have previously reported several methods of in-vitro immunization using different conditioned media. Here we introduce another method of in-vitro immunization using a mixture of three types of supernatant (thymocytes, and adherent and non-adherent splenocytes of mouse). Splenocytes were immunized in-vitro by a recombinant human growth hormone (rhGH) with the above conditioned media, and the results by ELISA showed a much higher optical density than the other in-vitro immunization methods that we had previously reported. Humoral responses as a result of in-vitro immunization to soluble antigens were easily confirmed by ELISA using the above-conditioned media. This finding indicates that any other conditions thought to be critical by other researches may not be essential for in-vitro immunization.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Bazo
/
Proteínas Recombinantes
/
Activación de Linfocitos
/
Linfocitos T
/
Adhesión Celular
/
Inmunización
/
Sustancias de Crecimiento
/
Ratones Endogámicos BALB C
/
Formación de Anticuerpos
/
Antígenos
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Korean Medical Science
Año:
1990
Tipo del documento:
Artículo
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