Survey of Rh Blood Type Phenotypes in Unpaid Blood Donors in Tongchuan Area of Shaanxi Province / 现代检验医学杂志

ABSTRACT

Objective To investigate the distribution of Rh blood type phenotype in unpaid blood donors in Tongchuan area and establish a Rh blood type phenotype database to provide a strong guarantee for clinical safe blood transfusion.Methods The Rh blood group antigens of 3 419 cases of blood donors samples were detected by the method of card column weak gel in the Tongchuan Blood Center from June 2015 to March 2017,and identification of RhD negative specimens were confirmed fordetermining the phenotype of its Rh blood group with indrect antiglobulin test.The cassification statistics were carried out and filed with computer.Rh distribution with other parts of the domestic distribution of blood type was compared using t test.Results The negative rate of RhD in 3 419 unrelated donors was 0.55％.The positive rates of other antigens in Rh were 99.45％,90.41％ in C,55.10％ in E,45.51％ in E and 92.19％ in E,respectively.D:0.921,C:0.673 7,c:0.320 7,E:0.275 5 and e:0.730 6.The distribution of Rh phenotype was:CCDee＞ CcDEe＞ CcDee＞ ccDEE＞ ccDEe＞ CcDEE＞ ccDee ＞ RhD negative.Statistical analysis showed that there was significant difference between the composition ratio of CCDee and ccDEE in Tongchuan and that in Nanning and Qiannan (x2 =21.552,P=0.016;x2=18.519,P=0.001).There was no significant difference between Shenyang,Xingtai and Tianjin Binhai (x2 =0.227～1.31,all P＞0.05).Conclusion The distribution of Rh phenotype in Tongchuan area has the characteristics of northern region[1～4].The RhD negative rate is in line with the distribution characteristics of Chinese Han population.Rh and Rh genotypes differ in southern and northern China,Phenotypic distribution of the investigation for the safety of blood transfusion is of great significance.