The oxidative damage on the proliferation of oral cancer SCC-25 cells / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6): 763-765, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-697421
ABSTRACT
Objective:
To study the exogenous oxidative stimuli on the mtDNA damage and proliferation of oral cancer SCC-25 cells.Methods:
SCC-25 cells and fibroblast cell line BJ cells were treated by H2O2 at 0 (the control),120,240,480 and 960 μmol/L respectively for 15 m-24 h respectively,mtDNA damage was measured by a supercoiling-sensitive qPCR method(ss-qPCR),cell proliferation was tested by MTT assay.Results:
Dose-dependent mtDNA damage was observed in SCC-25 cells treated by H2O2 at 120 μmol/L or above,and in BJ cells by H2O2 at 240 μmol/L or above,the mtDNA damage rate of both cells decreased 2-24 h after H2O2 treatment,in the 120 μmol/L group the damage rate reached to the control group.BJ cells showed less reaction to H2O2 stimulation than SCC-25 cells.Conclusion:
Oxidative stress may be related to the development of oral cancer.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Practical Stomatology
Año:
2017
Tipo del documento:
Artículo
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