Effect of microRNA-150 on proliferation,apoptosis,invasion and metastasis of epithelial ovarian cancer cells / 实用肿瘤学杂志

ABSTRACT

Objective The aim of this study was to investigate the expression of microRNA-150(miR-150)in human epi-thelial ovarian cancer cells and its effect on proliferation,apoptosis,invasion and metastasis of human epithelial ovarian cancer cells. Methods The expression level of miR-150 in cells from each treatment group was detected by Real-Time PCR(qRT-PCR);effects of proliferation,apoptosis,invasion and metastasis of epithelial ovarian cancer cells was investigated by MTT,flow cytometry, and transwell assays. Results Compared with normal ovarian epithelial cells(T29),the expression of miR-150 was significantly de-creased in epithelial ovarian cancer cells(A2780 and OVCAR3)(P<0. 01); After transfection miR-150 mimic,the expression of miR-150 in A2780 and OVCAR3 cells was significantly increased(P<0. 01);After 3 d of transfection,the OD values of the miR-150mimicgroup(A27801.12±0.03;OVCAR31.91±0.03)werelowerthanthatintheblankgroup(A27802.35±0.09;OVCAR32.63 ±0.07)and the miR-150 NC group(A27802.18 ±0.07;OVCAR32.43 ±0.11)(P<0.01);The apoptotic rate in the miR-150 mimic group(A278016. 10 ± 0. 58% ;OVCAR315. 16 ± 1. 30% ) were significantly increased when compared to the blank group(A278010. 07 ± 0. 66%;OVCAR33. 81 ± 0. 24%) and the miR -150 NC group(A278010. 36 ± 1. 08%;OVCAR34.89 ±0.07%)(P<0.01);The number of transmembrane cells in the miR-150 mimic group(A278038.67 ±2.03;OVCAR328. 67 ± 2. 03)was higher than that in the blank group(A278076. 30 ± 7. 45;OVCAR355. 67 ± 3. 18)and the miR-150 NC group(A278074. 33 ± 5. 78;OVCAR356. 33 ± 3. 84)(P<0. 01). Conclusion The decreased expression of miR-150 in epi-thelial cancer cells may be one of the mechanisms of proliferation,invasion and metastasis of epithelial ovarian cancer. Up-regulation of miR-150 may inhibit the proliferation of epithelial ovarian cancer cells and promote apoptosis to reduce the abilities of invasion and metastasis in epithelial ovarian cancer cells.