Effects of operation time of durotomy on acute spinal cord contusion / 西安交通大学学报(医学版)

ABSTRACT

Objective To study the effects of operation time of durotomy on acute spinal cord contusion. Methods Spinal cord contusion models were established in SD rats at T 9 by NYU-Ⅱ impactor.Durotomy was performed immediately(group C),1 h(group D),6 h(group E)and 12 h(group F)after spinal cord contusion, respectively.Rats in group B did not receive durotomy.Open-field locomotor function was evaluated using the Basso-Beattie-Bresnahan(BBB)locomotor rating scale at days 1,3,7,14,21 and 28 after injury in order to compare damage degree of the spinal cord.The percentage of cavity volume(CV%)and lesion length were calculated by"Imagej"software using serial sections stained by hematoxylin/eosin(HE).Results The BBB score in groups C and D was significantly better than that in group B(P ＜ 0.01);CV% and lesion length were significantly lower than those in group B(P＜0.01).The BBB score was higher in group E than in group B(P＜0.05),but lower than in groups C and D.Similarly,CV% was lower than that in group B(P＜0.05),but higher than that in groups C and D(P＜0.05).No difference was found between groups F and B in the BBB score,CV%or lesion length(P＞0.05).Conclusion Decompressive durotomy improved motor function recovery and reduced the damage of the spinal cord in the setting of a thoracic spinal cord contusion injury in SD rats within 6 h after injury.Durotomy 12 h after injury does not affect function outcomes or spinal cord injury after injury.