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Paired Primary and Metastatic Tumor Analysis of Somatic Mutations in Synchronous and Metachronous Colorectal Cancer / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment ; : 161-167, 2017.
Artículo en Inglés | WPRIM | ID: wpr-6985
ABSTRACT

PURPOSE:

Although the mutation status of KRAS is highly concordant in primary and metastatic lesions, it has not been generalized to other major pathway genes. MATERIALS AND

METHODS:

In this study, 41 genes were evaluated and the mutational profiles were compared in 46 colorectal cancer patients with paired surgical specimens of primary and metastatic lesions synchronous (n=27) and metachronous (n=19) lesions. A high-throughput mass spectrometry-based genotyping platform validated by orthogonal chemistry, OncoMap v.4.4, was used to evaluate the formalin-fixed, paraffin-embedded surgical specimens. The patients’ demographics, tumor characteristics, and microsatellite instability status were analyzed by a retrospective chart review.

RESULTS:

In this study,with OncoMap, mutationswere identified in 80.4% of patientswith the following frequency KRAS (39.1%), TP53 (28.3%), APC (28.3%), PIK3CA (6.5%), BRAF (6.5%), and NRAS (4.3%). Although 19.6% (9/46) of the patients showed no gene mutations, 43.5% (20/46) and 37.0% (17/46) had mutations in one and two or more genes, respectively. The synchronous and metachronous lesions showed similar mutational profiles. Paired samples between primary and metastatic tumors differed in 7.4% (2/27) and 10.5% (2/19) for synchronous and metachronous according to OncoMap.

CONCLUSION:

These findings indicate the major pathway genes, including KRAS, TP53, APC, PIK3CA, BRAF, and NRAS, are often concordant between the primary and metastatic lesions regardless of the temporal relationship of metastasis.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Neoplasias Colorrectales / Química / Demografía / Estudios Retrospectivos / Genómica / Inestabilidad de Microsatélites / Metástasis de la Neoplasia Tipo de estudio: Estudio observacional / Estudio pronóstico / Factores de riesgo Límite: Humanos Idioma: Inglés Revista: Cancer Research and Treatment Año: 2017 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Neoplasias Colorrectales / Química / Demografía / Estudios Retrospectivos / Genómica / Inestabilidad de Microsatélites / Metástasis de la Neoplasia Tipo de estudio: Estudio observacional / Estudio pronóstico / Factores de riesgo Límite: Humanos Idioma: Inglés Revista: Cancer Research and Treatment Año: 2017 Tipo del documento: Artículo