Differential Expression of miRNA in Breast Cancer and the Significance in Prognosis / 医学研究杂志

ABSTRACT

Objective To explore changes in the expression of MicroRNAs related to prognosis in breast cancer tissue.Methods Microarray data of breast cancer tissue were downloaded from the GEO database,including MicroRNAs profilings of 10 year distant-relapse free survival and the controls.The differentially-expressed MicroRNAs were identified use GEO2R tool.The upstream transcription factor(TF) and downstream target genes of differentially-expressed MicroRNAs were investigated with prediction websites.Function and pathway of target genes were annotated by DAVID.Regulating network of TF-miRNA-target gene were constructed by cytoscape.Results A total of 5 miRNAs were differentially expressed (3 up-and 2 down-regulated).5 upstream TFs and 154 downstream target genes were found respectively.Functional enrichment analysis indicated that negative regulation of BMP signaling pathway,cytoskeleton organization,signaling by TGF beta,signaling by Rho GTPases were involved.Conclusion hsa-miR-210,hsa-miR-33a,hsa-miR-32,hsa-miR-135a,hsa-miR-30a-3p were the differentially-expressed miRNAs relevant to prognosis of breast cancer.Our study systemically characterize miRNAs expression changes using bioinformatics methods to the benefit of the diagnosis and treatment for breast caner prognosis.